Ashutosh Singh | Virology | Best Researcher Award

Published on by Global Diseases Research

Mr. Ashutosh Singh | Virology | Best Researcher Award

Phd Scholar at VIT Bhopal University, India

Ashutosh Kumar Singh is a dedicated biotechnology researcher specializing in virology, computational biology, and molecular diagnostics. His academic and professional journey reflects a strong commitment to advancing the understanding of viral pathogens and their impact on human and environmental health. He has developed expertise in laboratory techniques such as droplet digital PCR, agarose gel electrophoresis, microscopy, primer designing, and molecular docking using advanced computational tools. Through his work, he has contributed to international publications and actively participated in conferences, presenting his research on wastewater-based epidemiology, viral detection, and structural analysis of pathogens. His interest extends to the integration of computational and experimental approaches, enabling him to explore host-pathogen interactions and the role of environmental surveillance in public health. With a vision to bridge laboratory science with real-world challenges, Ashutosh Kumar Singh continues to grow as a researcher committed to innovation, global health security, and sustainable disease monitoring strategies.

Professional Profile

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Education

Ashutosh Kumar Singh has pursued a comprehensive academic journey in biotechnology, beginning with foundational studies in biological sciences and later advancing into specialized training in virology, molecular diagnostics, and computational biology. His education equipped him with a strong grounding in laboratory techniques, analytical skills, and the theoretical knowledge required to address complex biological questions. During his higher studies, he explored cutting-edge tools in molecular biology, including droplet digital PCR, primer design, molecular docking, and bioinformatics. His academic progression reflects a balance between experimental practice and computational applications, preparing him to address multidisciplinary challenges in biotechnology. Through coursework, seminars, and research-based projects, he developed critical thinking and problem-solving abilities, further enriched by his participation in workshops and scientific discussions. His educational journey has cultivated a deep interest in global health, virology, and environmental monitoring, establishing a strong foundation for his ongoing research and professional growth in the field of biotechnology.

Experience

Ashutosh Kumar Singh has gained substantial experience through research, publications, and active participation in scientific forums. He has presented posters and delivered talks at national and international conferences, focusing on the detection and surveillance of enteric viruses in environmental and clinical settings. His work highlights the application of droplet digital PCR in tracking pathogens such as norovirus, rotavirus, and astrovirus. He has collaborated on multiple research studies that investigate viral epidemiology, genotypic diversity, and host-pathogen interactions. Beyond bench work, he has engaged with computational approaches, applying molecular docking, phylogenetics, and structural analysis to better understand viral mechanisms. His contributions include several published research articles in reputed journals, showcasing his ability to connect environmental surveillance with public health outcomes. His experience reflects a blend of experimental expertise, computational analysis, and active engagement with the scientific community, enabling him to make meaningful contributions to virology, biotechnology, and global health research.

Research Focus

Ashutosh Kumar Singh’s research focuses on the epidemiology, genetic diversity, and environmental monitoring of infectious pathogens with significant public health impact. His studies investigate enteric viruses such as noroviruses and rotaviruses, applying advanced molecular tools like droplet digital PCR to enhance pathogen detection in wastewater and community samples. Singh’s work also extends to bacterial diseases including scrub typhus and genital tuberculosis, addressing their role in reproductive health and disease transmission. By conducting systematic reviews and meta-analyses, he synthesizes existing evidence to provide a clearer understanding of disease patterns and clinical implications. His interdisciplinary research bridges virology, microbiology, and epidemiology, emphasizing the importance of One Health perspectives in tackling emerging infections. Singh is particularly interested in integrating laboratory diagnostics with surveillance frameworks, enabling early detection and prevention strategies. His research ultimately aims to inform healthcare policies, strengthen global health security, and reduce the burden of infectious diseases worldwide.

Publication Top Notes

Title: Investigating the Presence of Rotavirus in Wastewater Samples of Bhopal Region, India, by Utilizing Droplet Digital Polymerase Chain Reaction
Authors: RK Nema, AK Singh, J Nagar, B Prajapati, M Sikenis, S Singh, V Diwan, et al.
Journal: Cureus, 16(4), 2024
Summary: This study applied droplet digital PCR (ddPCR) to detect rotavirus in wastewater samples from Bhopal, India. The findings highlight the importance of wastewater surveillance in monitoring viral pathogens and supporting public health strategies.

Title: Deciphering the Genotypic Diversity and Epidemiology of Scrub Typhus Genotypes in India: A Systematic Review and Meta-Analysis
Authors: RK Nema, AK Singh, D Raj, P Gupta, RR Tiwari, S Purwar
Journal: Microbial Pathogenesis, Article 107182, 2024
Summary: This systematic review and meta-analysis explored the genetic diversity of Orientia tsutsugamushi genotypes in India. It provides insights into scrub typhus epidemiology and stresses the need for genotype-specific diagnostic and preventive strategies.

Title: Protocol for Detection of Pathogenic Enteric RNA Viruses by Regular Monitoring of Environmental Samples from Wastewater Treatment Plants Using Droplet Digital PCR
Authors: RK Nema, S Singh, AK Singh, DK Sarma, V Diwan, RR Tiwari, RK Mondal, et al.
Journal: Science in One Health, 3, 100080, 2024
Summary: This article presents a standardized ddPCR-based protocol for detecting enteric RNA viruses in wastewater treatment plants. The method improves sensitivity and accuracy, aiding in early outbreak detection and public health preparedness.

Title: The Evolving Landscape of Norovirus GII Genotypes in Asia: A Systematic Review and Meta-Analysis
Authors: AK Singh, J Nagar, A Tandekar, S Singh, V Diwan, GC Ravindran, et al.
Journal: Journal of Clinical Virology, Article 105809, 2025
Summary: This meta-analysis examined Norovirus GII genotype diversity across Asia. The findings highlight emerging strains, epidemiological trends, and implications for vaccine development and regional disease control.

Title: Genital Tuberculosis: A Silent Contributor to Infertility and Adverse Pregnancy Outcomes
Authors: M Sikenis, B Prajapati, AK Singh, K Yadav, A Rahman, MK Pandey, et al.
Journal: Journal of Reproductive Healthcare and Medicine, 5(17), 2024
Summary: This review highlights the overlooked role of genital tuberculosis in infertility and pregnancy complications. It emphasizes the importance of improved screening and diagnostic awareness in reproductive healthcare.

Title: Detection of Noroviruses GII in Wastewater Samples of Bhopal, India, Using Droplet Digital PCR
Authors: RK Nema, J Nagar, AK Singh, A Tandekar, S Singh, A Rahman, et al.
Journal: medRxiv (Preprint), 2023
Summary: This preprint reports the detection of Norovirus GII in wastewater samples from Bhopal using ddPCR. The study underscores the value of environmental surveillance in tracking viral spread and assessing community health risks.

Conclusion

Ashutosh Kumar Singh embodies the qualities of a researcher committed to scientific innovation, interdisciplinary collaboration, and public health advancement. His journey demonstrates a balance of academic rigor, laboratory expertise, and computational proficiency, positioning him as a promising contributor in the fields of biotechnology and virology. His dedication to understanding viral pathogens and developing sensitive diagnostic methods reflects his vision of integrating science with practical solutions for global health challenges. With multiple publications, active conference participation, and hands-on experience in advanced molecular techniques, he has established a strong foundation for impactful contributions to research and society. His work in wastewater-based epidemiology underscores the importance of environmental monitoring in preventing and controlling outbreaks, while his computational insights strengthen the understanding of viral evolution and host interactions. Moving forward, his focus remains on expanding innovative research, building global collaborations, and applying biotechnology for the betterment of human and environmental health.

Marie Amougou Atsama | Gastroenteritis | Best Researcher Award

Published on by Global Diseases Research

Dr. Marie Amougou Atsama | Gastroenteritis | Best Researcher Award 

Research Officer at Research Center on Emerging and Re-merging ,Cameroon

Dr. Amougou Atsama Marie is a highly accomplished public health manager and virologist with extensive expertise in research, biosafety, and disease prevention. She currently serves as Biosafety Officer and Public Health Manager at the Research Center for Emerging and Re-Emerging Infectious Diseases in Yaoundé, Cameroon. With a strong focus on viral hepatitis and other infectious diseases, she has led numerous initiatives aimed at prevention, surveillance, and control within Central Africa. Her work spans laboratory research, public health program coordination, and stakeholder engagement at national and regional levels. Dr. Marie has played a key role in developing diagnostic protocols, improving health systems, and fostering community-based prevention strategies. Her leadership extends to advocacy, capacity building, and integrating innovative approaches, such as the use of improved traditional medicine in disease management. Dedicated, results-oriented, and deeply committed to public health, she continues to advance impactful research and healthcare improvement in underserved communities.

Professional Profile

Scopus

Education

Dr. Amougou Atsama Marie possesses a strong academic background in virology, biotechnology, and biochemistry, built through rigorous studies and advanced research training. She completed her doctoral and postdoctoral work at the renowned Centre Pasteur of Cameroon, where she specialized in the molecular and epidemiological aspects of various viral pathogens. She earned her master’s degree in biotechnology and development from the University of Yaoundé I, developing a foundation in molecular biology, diagnostics, and public health applications. Her undergraduate degree in biochemistry further strengthened her understanding of biological systems and analytical methods. Over the years, she has complemented her formal education with specialized certifications in good clinical laboratory practice, human research ethics, quality control management, and advanced genomic sequencing techniques. These academic achievements, coupled with hands-on research experience, have equipped her with both theoretical knowledge and practical expertise, enabling her to design, implement, and oversee complex public health and laboratory research programs effectively.

Experience

Dr. Amougou Atsama Marie has established herself as a leader in public health management and virology through diverse roles in research, biosafety, and quality control. As Biosafety and Research Officer, she has coordinated regional efforts for the elimination of viral hepatitis B and C across multiple Central African countries, facilitating capacity-building workshops and advocacy programs. She has developed and optimized diagnostic protocols for a range of viruses, including hepatitis B, C, D, E, Dengue, Chikungunya, and Zika, while implementing robust surveillance and monitoring systems. Her work involves close collaboration with national and international health partners to strengthen healthcare delivery and integrate innovative solutions into disease prevention strategies. She has also led research on vaccine response, genetic diversity of hepatitis viruses, and the integration of traditional medicine in treatment. In addition to her technical expertise, she actively engages in community education, raising awareness about infectious disease prevention among students and the public.

Awards and Honors

Dr. Amougou Atsama Marie has received multiple recognitions for her outstanding contributions to virology research, biosafety, and public health initiatives. Her leadership in hepatitis elimination programs across Central Africa has earned her commendations from national health authorities and regional stakeholders. She has been honored for her innovative approaches in integrating traditional medicine into viral hepatitis management and for developing advanced diagnostic protocols for various infectious diseases. Her work in facilitating high-impact workshops, mentoring healthcare professionals, and contributing to national health guidelines has been acknowledged by both academic and public health institutions. She is respected for her role in promoting cross-border collaboration in disease control and for her dedication to building local capacity in laboratory diagnostics and biosafety management. Through these recognitions, Dr. Marie’s influence extends beyond research, highlighting her as a key figure in advancing evidence-based, community-centered health solutions in Cameroon and the broader Central African region.

Research Focus

Dr. Amougou Atsama Marie’s research centers on the prevention, diagnosis, and control of viral hepatitis and other emerging infectious diseases. She is particularly focused on understanding the genetic diversity of hepatitis viruses and its impact on vaccine response and treatment outcomes. Her work involves developing and optimizing molecular and serological diagnostic protocols for a wide range of viruses, including arboviruses such as Dengue, Chikungunya, and Zika. She also investigates strategies to integrate traditional medicine into viral hepatitis management, aiming to improve accessibility and cultural acceptance of treatment in local communities. Dr. Marie is actively involved in operational research that strengthens health systems, enhances biosafety measures, and improves outbreak preparedness. Her approach combines laboratory-based research with community engagement, ensuring that scientific advancements translate into practical, sustainable health solutions. By bridging research, policy, and public health action, she contributes to regional and global efforts to control infectious diseases effectively.

Publication Top Notes

Title: First molecular detection of five gastroenteritis viruses in untreated wastewater and impact of seasonal change on their distribution in Cameroon
Journal: One Health (2025)
Summary:
This study presents the first molecular evidence of five major gastroenteritis-causing viruses detected in untreated wastewater in Cameroon. Using molecular techniques, researchers identified the presence of these pathogens and assessed how their distribution varies across different seasons. The findings reveal a clear influence of seasonal patterns on virus prevalence, highlighting the importance of wastewater surveillance as an early warning system for community health risks. The research supports the One Health approach by linking environmental monitoring to public health interventions, offering valuable insights for disease prevention and control strategies in the region.

Conclusion

Dr. Amougou Atsama Marie embodies a rare combination of scientific expertise, leadership, and dedication to public health improvement. Her career reflects a consistent commitment to advancing research, enhancing diagnostic capabilities, and promoting evidence-based interventions for infectious disease control. Through her work in laboratory innovation, biosafety, and community engagement, she has significantly contributed to the fight against viral hepatitis and other infectious threats in Central Africa. Her ability to collaborate with diverse stakeholders, from policymakers to grassroots organizations, underscores her holistic approach to healthcare solutions. By integrating modern scientific methods with culturally sensitive strategies, she ensures that her work is both impactful and sustainable. Dr. Marie’s professional journey serves as an inspiring example of how research excellence and public health service can intersect to create lasting change, improving the lives of individuals and strengthening health systems across regions.