Ashutosh Singh | Virology | Best Researcher Award

Published on by Global Diseases Research

Mr. Ashutosh Singh | Virology | Best Researcher Award

Phd Scholar at VIT Bhopal University, India

Ashutosh Kumar Singh is a dedicated biotechnology researcher specializing in virology, computational biology, and molecular diagnostics. His academic and professional journey reflects a strong commitment to advancing the understanding of viral pathogens and their impact on human and environmental health. He has developed expertise in laboratory techniques such as droplet digital PCR, agarose gel electrophoresis, microscopy, primer designing, and molecular docking using advanced computational tools. Through his work, he has contributed to international publications and actively participated in conferences, presenting his research on wastewater-based epidemiology, viral detection, and structural analysis of pathogens. His interest extends to the integration of computational and experimental approaches, enabling him to explore host-pathogen interactions and the role of environmental surveillance in public health. With a vision to bridge laboratory science with real-world challenges, Ashutosh Kumar Singh continues to grow as a researcher committed to innovation, global health security, and sustainable disease monitoring strategies.

Professional Profile

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Education

Ashutosh Kumar Singh has pursued a comprehensive academic journey in biotechnology, beginning with foundational studies in biological sciences and later advancing into specialized training in virology, molecular diagnostics, and computational biology. His education equipped him with a strong grounding in laboratory techniques, analytical skills, and the theoretical knowledge required to address complex biological questions. During his higher studies, he explored cutting-edge tools in molecular biology, including droplet digital PCR, primer design, molecular docking, and bioinformatics. His academic progression reflects a balance between experimental practice and computational applications, preparing him to address multidisciplinary challenges in biotechnology. Through coursework, seminars, and research-based projects, he developed critical thinking and problem-solving abilities, further enriched by his participation in workshops and scientific discussions. His educational journey has cultivated a deep interest in global health, virology, and environmental monitoring, establishing a strong foundation for his ongoing research and professional growth in the field of biotechnology.

Experience

Ashutosh Kumar Singh has gained substantial experience through research, publications, and active participation in scientific forums. He has presented posters and delivered talks at national and international conferences, focusing on the detection and surveillance of enteric viruses in environmental and clinical settings. His work highlights the application of droplet digital PCR in tracking pathogens such as norovirus, rotavirus, and astrovirus. He has collaborated on multiple research studies that investigate viral epidemiology, genotypic diversity, and host-pathogen interactions. Beyond bench work, he has engaged with computational approaches, applying molecular docking, phylogenetics, and structural analysis to better understand viral mechanisms. His contributions include several published research articles in reputed journals, showcasing his ability to connect environmental surveillance with public health outcomes. His experience reflects a blend of experimental expertise, computational analysis, and active engagement with the scientific community, enabling him to make meaningful contributions to virology, biotechnology, and global health research.

Research Focus

Ashutosh Kumar Singh’s research focuses on the epidemiology, genetic diversity, and environmental monitoring of infectious pathogens with significant public health impact. His studies investigate enteric viruses such as noroviruses and rotaviruses, applying advanced molecular tools like droplet digital PCR to enhance pathogen detection in wastewater and community samples. Singh’s work also extends to bacterial diseases including scrub typhus and genital tuberculosis, addressing their role in reproductive health and disease transmission. By conducting systematic reviews and meta-analyses, he synthesizes existing evidence to provide a clearer understanding of disease patterns and clinical implications. His interdisciplinary research bridges virology, microbiology, and epidemiology, emphasizing the importance of One Health perspectives in tackling emerging infections. Singh is particularly interested in integrating laboratory diagnostics with surveillance frameworks, enabling early detection and prevention strategies. His research ultimately aims to inform healthcare policies, strengthen global health security, and reduce the burden of infectious diseases worldwide.

Publication Top Notes

Title: Investigating the Presence of Rotavirus in Wastewater Samples of Bhopal Region, India, by Utilizing Droplet Digital Polymerase Chain Reaction
Authors: RK Nema, AK Singh, J Nagar, B Prajapati, M Sikenis, S Singh, V Diwan, et al.
Journal: Cureus, 16(4), 2024
Summary: This study applied droplet digital PCR (ddPCR) to detect rotavirus in wastewater samples from Bhopal, India. The findings highlight the importance of wastewater surveillance in monitoring viral pathogens and supporting public health strategies.

Title: Deciphering the Genotypic Diversity and Epidemiology of Scrub Typhus Genotypes in India: A Systematic Review and Meta-Analysis
Authors: RK Nema, AK Singh, D Raj, P Gupta, RR Tiwari, S Purwar
Journal: Microbial Pathogenesis, Article 107182, 2024
Summary: This systematic review and meta-analysis explored the genetic diversity of Orientia tsutsugamushi genotypes in India. It provides insights into scrub typhus epidemiology and stresses the need for genotype-specific diagnostic and preventive strategies.

Title: Protocol for Detection of Pathogenic Enteric RNA Viruses by Regular Monitoring of Environmental Samples from Wastewater Treatment Plants Using Droplet Digital PCR
Authors: RK Nema, S Singh, AK Singh, DK Sarma, V Diwan, RR Tiwari, RK Mondal, et al.
Journal: Science in One Health, 3, 100080, 2024
Summary: This article presents a standardized ddPCR-based protocol for detecting enteric RNA viruses in wastewater treatment plants. The method improves sensitivity and accuracy, aiding in early outbreak detection and public health preparedness.

Title: The Evolving Landscape of Norovirus GII Genotypes in Asia: A Systematic Review and Meta-Analysis
Authors: AK Singh, J Nagar, A Tandekar, S Singh, V Diwan, GC Ravindran, et al.
Journal: Journal of Clinical Virology, Article 105809, 2025
Summary: This meta-analysis examined Norovirus GII genotype diversity across Asia. The findings highlight emerging strains, epidemiological trends, and implications for vaccine development and regional disease control.

Title: Genital Tuberculosis: A Silent Contributor to Infertility and Adverse Pregnancy Outcomes
Authors: M Sikenis, B Prajapati, AK Singh, K Yadav, A Rahman, MK Pandey, et al.
Journal: Journal of Reproductive Healthcare and Medicine, 5(17), 2024
Summary: This review highlights the overlooked role of genital tuberculosis in infertility and pregnancy complications. It emphasizes the importance of improved screening and diagnostic awareness in reproductive healthcare.

Title: Detection of Noroviruses GII in Wastewater Samples of Bhopal, India, Using Droplet Digital PCR
Authors: RK Nema, J Nagar, AK Singh, A Tandekar, S Singh, A Rahman, et al.
Journal: medRxiv (Preprint), 2023
Summary: This preprint reports the detection of Norovirus GII in wastewater samples from Bhopal using ddPCR. The study underscores the value of environmental surveillance in tracking viral spread and assessing community health risks.

Conclusion

Ashutosh Kumar Singh embodies the qualities of a researcher committed to scientific innovation, interdisciplinary collaboration, and public health advancement. His journey demonstrates a balance of academic rigor, laboratory expertise, and computational proficiency, positioning him as a promising contributor in the fields of biotechnology and virology. His dedication to understanding viral pathogens and developing sensitive diagnostic methods reflects his vision of integrating science with practical solutions for global health challenges. With multiple publications, active conference participation, and hands-on experience in advanced molecular techniques, he has established a strong foundation for impactful contributions to research and society. His work in wastewater-based epidemiology underscores the importance of environmental monitoring in preventing and controlling outbreaks, while his computational insights strengthen the understanding of viral evolution and host interactions. Moving forward, his focus remains on expanding innovative research, building global collaborations, and applying biotechnology for the betterment of human and environmental health.

Sadiq Azam | Clinical Microbiology | Best Researcher Award

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Assoc. Prof. Dr. Sadiq Azam | Clinical Microbiology | Best Researcher Award

Associate Professor at University of Peshawsar, Pakistan

Dr. Sadiq Azam is an accomplished Associate Professor at the University of Peshawar, specializing in microbiology and biotechnology. With over a decade of academic and research experience, he has significantly contributed to understanding antibiotic resistance in pathogenic bacteria and the antimicrobial properties of medicinal plants. Dr. Azam began his academic career at Bacha Khan University and soon joined the University of Peshawar, where he has progressed through various academic ranks. His research includes several completed projects funded by the Higher Education Commission (HEC) and the Directorate of Science and Technology (DoST), focusing on bacteriological profiling, phytochemical analysis, and molecular characterization of drug-resistant pathogens. A certified supervisor, he has mentored numerous M.Phil, PhD, and undergraduate students. He is an active member of academic boards across various institutions and plays a vital role in curriculum development, examination coordination, and teaching innovation. Dr. Azam is fluent in English, Urdu, and Pashto.

Professional Profiles

Scopus

Education 

Dr. Sadiq Azam earned his PhD in Biotechnology from the University of Peshawar, where he had also completed his Bachelor’s in Biotechnology. Prior to his university education, he studied pre-medical sciences at Nisar Shaheed Degree College, Resalpur. His academic journey began with a foundation in science at Government High School, Swabi. Throughout his education, he focused on understanding biological systems and laboratory methodologies, which laid the groundwork for his later research in antimicrobial resistance, pathogenic microorganisms, and medicinal plant compounds. His doctoral work, supported by a prestigious HEC Indigenous Scholarship, marked the beginning of a research career centered on the intersection of microbiology, molecular biology, and pharmacognosy. The academic rigor and scientific training received throughout his education have shaped his multifaceted expertise in clinical microbiology and applied biotechnology. His educational foundation continues to support his teaching, research supervision, and grant-funded investigations in Pakistan’s higher education and health sectors.

Experience 

Dr. Azam’s professional journey began in 2011 as Assistant Professor at Bacha Khan University, Charsadda. He joined the University of Peshawar, where he advanced from Lecturer to Assistant Professor and now serves as an Associate Professor in the Centre for Biotechnology and Microbiology. With over 13 years of teaching and research experience, he has been involved in undergraduate and postgraduate education, thesis supervision, and lab-based training. Beyond teaching, he has led and collaborated on national research projects focused on phytochemical screening and antibiotic resistance. Dr. Azam has played active administrative roles, including BS program coordination and examination management. He is also a member of several Boards of Studies across universities in Khyber Pakhtunkhwa, contributing to academic planning and quality assurance. His career highlights a balance of academic instruction, impactful research, institutional service, and mentorship in the fields of microbiology, biotechnology, and infectious disease research.

Awards and Honors 

Dr. Sadiq Azam was awarded the prestigious HEC Indigenous Scholarship for his M.Phil to PhD studies, recognizing his academic excellence and potential in scientific research. This scholarship provided vital support for his doctoral work in biotechnology at the University of Peshawar and laid the foundation for a research career focused on combating antimicrobial resistance and exploring plant-based therapeutic alternatives. In addition to academic funding, Dr. Azam has successfully secured competitive research grants from Pakistan’s Higher Education Commission (HEC) and the Directorate of Science and Technology (DoST). These projects, as both Principal and Co-Principal Investigator, reflect national recognition of his expertise and contributions. His continued involvement in funded research projects underscores his credibility and leadership in the scientific community. His honors not only represent personal achievements but also contribute to the growth of biotechnology research capacity in Pakistan. He remains committed to advancing microbiological science through innovation and collaboration.

Research Focus 

Dr. Sadiq Azam’s research centers on bacteriological profiling, molecular genetics of antibiotic resistance, and the pharmacological evaluation of medicinal plants. His primary focus is the identification of resistance genes in clinical isolates, including extended-spectrum β-lactamase (ESBL) and carbapenemase-producing Escherichia coli and Klebsiella pneumoniae. He employs molecular characterization techniques such as PCR, MIC testing, analytical profiling, and in-silico drug design to combat emerging bacterial threats. A parallel area of interest lies in the bioassay screening and phytochemical analysis of traditional medicinal plants like Silene conoidea, Sarcococca saligna, and Pinus wallichiana, aiming to discover novel antimicrobial compounds. His integrated approach bridges clinical microbiology with natural product chemistry, addressing global challenges in antibiotic resistance and infectious disease management. Through research supervision and project leadership, he actively contributes to building Pakistan’s research capacity in biotechnology, while aligning with global health priorities on antimicrobial stewardship and sustainable therapeutics.

Publication Top Notes

Title: Design and Validation of a Polyunit Epitope Vaccine Against Enterococcus faecium
Journal: Journal of Computational Biophysics and Chemistry
Summary:
This study likely focuses on the computational design and validation of a multi-epitope subunit vaccine targeting E. faecium, a notorious multidrug-resistant pathogen. The vaccine design may have used immunoinformatics tools to identify epitopes and predict immunogenicity.

Title: Towards Precision Epitopes Based Vaccine Against Enterococcus faecalis by Integrating Vaccinomics, Reverse Vaccinology and Biophysics Approaches
Journal: Biochemistry and Biophysics Reports
Summary:
The paper integrates vaccinomics and reverse vaccinology to identify precision epitopes for a vaccine targeting E. faecalis. It likely combines computational prediction, immunological profiling, and structural biophysics to develop a targeted vaccine strategy.

Title: Current Pattern of Antibiotic Resistance and Molecular Characterization of Virulence Genes in Klebsiella pneumoniae Obtained from Urinary Tract Infection (UTIs) Patients, Peshawar
Journal: PLOS ONE
Summary:
This research investigates antibiotic resistance patterns and the genetic basis of virulence in K. pneumoniae strains isolated from UTI patients in Peshawar. Likely includes PCR-based detection of virulence genes and antibiotic susceptibility profiling.

Title: Elucidating the Resistance Mechanisms and Binding Pattern of Novel Oxa-48-like Carbapenemases Covalent Inhibitors: A Hybrid Experimental and In Silico Approach
Journal: Journal of Molecular Structure
Summary:
The article explores how novel covalent inhibitors interact with Oxa-48-like carbapenemases—enzymes that confer resistance to carbapenem antibiotics. Uses both computational modeling and experimental data to understand resistance and inhibitor design.

Title: Green Synthesis and Characterisation of Desmodium podocarpum-Mediated Silver Nanoparticles and Their Biological Activities
Journal: Natural Product Research
Summary:
Focuses on the eco-friendly synthesis of silver nanoparticles using Desmodium podocarpum plant extract. Likely includes characterization (UV-Vis, SEM, FTIR) and evaluation of antimicrobial or antioxidant properties.

Conclusion 

Dr. Sadiq Azam stands as a leading academic and researcher in Pakistan’s biotechnology and microbiology landscape. His multidisciplinary expertise combines molecular microbiology, antimicrobial resistance research, and natural product pharmacology. With a strong academic foundation and over a decade of professional experience, he has contributed significantly to both science and education through impactful research, project leadership, and student mentorship. His work aligns with urgent global health issues such as drug-resistant infections and the search for alternative therapies. He has successfully led nationally funded projects and fostered collaborations across institutions. As an educator, Dr. Azam is deeply engaged in curriculum design, teaching innovation, and research supervision, shaping the next generation of scientists. His fluency in English, Urdu, and Pashto enables effective communication and outreach across diverse academic and community settings. Dr. Azam exemplifies the role of a dedicated researcher and educator committed to scientific advancement, public health improvement, and academic excellence in Pakistan.

Ragala Venkata Nandha | Clinical Microbiology | Best Scholar Award

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Mr. Ragala Venkata Nandha | Clinical Microbiology | Best Scholar Award 

Research Scholar at University of Madras, India

Ragala Venkata Nandha is a passionate young researcher currently pursuing his PhD in Medical Microbiology at Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Chennai. With a strong foundation in microbiological sciences, his work centers on antimicrobial resistance and advanced diagnostic techniques. He holds a Master’s degree in Medical Microbiology and a Bachelor’s in Medical Laboratory Technology. Nandha has contributed to multiple research projects funded by CMRG and RUSA 2.0, addressing clinical microbiology and drug resistance mechanisms. His scientific publications and conference presentations underscore his active engagement in cutting-edge research. As a first and co-author, he has contributed to reputed international journals and book chapters. He is a contributing member of the American Society for Microbiology and actively participates in conferences and workshops. Known for his practical expertise in molecular biology and microbial diagnostics, Nandha is committed to advancing solutions for combating infectious diseases and public health threats.

Professional Profiles

Scopus

Education

Ragala Venkata Nandha is academically trained in the field of microbiology and medical laboratory technology. He is currently enrolled in a PhD program in Medical Microbiology at the Department of Microbiology, Dr. ALM PG IBMS, University of Madras, where he is investigating antimicrobial resistance mechanisms. Prior to his doctoral studies, he earned his Master of Science (M.Sc.) in Medical Microbiology from the same institute, where he conducted research on probiotic enhancement. His undergraduate studies were in Medical Laboratory Technology (B.Sc. MLT) at Dr. NTR University, Vijayawada, Andhra Pradesh. Throughout his academic journey, Nandha has consistently focused on clinical microbiology, diagnostics, and molecular biology. His education has provided him with solid theoretical grounding and extensive hands-on laboratory experience in handling clinical specimens, performing molecular diagnostics, and applying modern research methodologies to real-world medical problems. His academic foundation supports his current research in combating multidrug-resistant pathogens.

Experience

Ragala Venkata Nandha has gained practical and research experience through his roles as a Project Fellow in two major funded research projects. He is currently working as a CMRG Project Fellow in the Department of Genetics at Dr. ALM PG IBMS, University of Madras, where he is developing a multiplex LAMP assay for rapid detection of resistance genes in colistin-resistant Enterobacteriaceae. Prior to this, he served as a RUSA 2.0 Project Fellow, focusing on the neuroimmunoendocrine effects of natural resources in neurodegenerative diseases. His experience includes microbial culture, molecular techniques such as PCR and sequencing, antimicrobial susceptibility testing, and analysis of co-resistance patterns. He also has hands-on training in animal handling and diagnostic laboratory workflows. These roles have enhanced his understanding of translational research and enabled him to contribute significantly to microbiology and infectious disease research at the institutional and national levels.

Research Focus 

Ragala Venkata Nandha’s research is centered on antimicrobial resistance (AMR) and molecular diagnostics. His current PhD project involves the isolation and molecular profiling of carbapenem- and colistin-resistant Gram-negative bacteria from clinical samples. The research aims to understand co-resistance patterns and the evolutionary mechanisms underlying these pathogens. In the CMRG project, he is developing a multiplex LAMP assay to detect mcr-1 to mcr-9 genes responsible for colistin resistance. His postgraduate work explored the prebiotic potential of spinach on probiotic growth, contributing to the understanding of gut microbiota modulation. He has also studied nanotechnology-based antimicrobial agents like eco-friendly silver nanoparticles. His work integrates microbiology, molecular biology, and bioinformatics to address critical healthcare challenges. With expertise in techniques like real-time PCR, Sanger sequencing, and bioinformatics analysis, his goal is to innovate diagnostics and therapeutic strategies for combating drug-resistant infections and improving clinical outcomes.

Publication Top Notes

Title: Advancing beyond conventional vaccines: The potential of next-generation vaccines in combatting antibiotic-resistant bacteria

Journal: Indian Journal of Medical Microbiology

Summary

This article explores the emerging landscape of next-generation vaccines designed to address the growing threat of antibiotic-resistant bacteria. Unlike traditional vaccines, which primarily rely on attenuated or inactivated pathogens, next-gen vaccines leverage novel platforms such as mRNA, recombinant proteins, vector-based systems, and nanoparticle technologies. The paper highlights how these vaccines offer greater specificity, faster development timelines, and adaptability against evolving bacterial strains. It also examines clinical trials, current research trends, and regulatory challenges, emphasizing the crucial role these vaccines play in reducing reliance on antibiotics and slowing antimicrobial resistance (AMR) worldwide.

Conclusion

Ragala Venkata Nandha represents a new generation of focused, skilled, and research-driven microbiologists working at the intersection of clinical diagnostics and antimicrobial resistance. His academic foundation, complemented by ongoing doctoral research, showcases a strong commitment to scientific inquiry and innovation. From molecular biology and nanotechnology to clinical microbiology and bioinformatics, his multidisciplinary approach addresses urgent public health concerns. His contributions as a project fellow, author, and conference presenter reflect both technical expertise and scientific maturity. As a member of international scientific communities and contributor to peer-reviewed literature, he continues to build a credible research profile. With promising achievements in diagnostics development and microbial resistance studies, Nandha is well-positioned to make a lasting impact in translational microbiological research. His journey reflects a dedication not only to academic excellence but also to solving real-world health challenges through research, collaboration, and innovation. He is a valuable asset to the scientific and medical research community.